L4402

Lysostaphin is supplied as a single polypeptide chain without the signal and propeptide regions.

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L4402

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Lysostaphin from Staphylococcus staphylolyticus

BioUltra, lyophilized powder, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

Synonym:Glycyl-glycine Endopeptidase
CAS Number:9011-93-2
Enzyme Commission (EC) Number:3.4.24.75   ( BRENDA | IUBMB )
MDL number:MFCD00131556

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Description

Biochem/physiol ActionsLysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5
General descriptionPackage size based on protein content
Preparation NoteAffinity purified
Unit DefinitionOne unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.
Physical formContains potassium phosphate buffer salts and sodium chloride

Properties

gradeBioUltra
assay≥97% (SDS-PAGE)
formlyophilized powder
compositionProtein, 40-60% biuret
EQP levelPremium
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements22-24/25
WGK Germany3
RTECSOL5985000

References

referenceBoneca, et al., Characterization of Staphylococcus aureus cell wall glycan strands, evidence for a new β-N-acetyl glucosaminidase activity. J. Biol. Chem. 275, 9910-9918, (2000)
 Trayer, H. R., and Buckley, C. E., J. Biol. Chem. 245, 4842-4846 , (1970)
 Browder, H.P., et al., Biochem. Biophys. Res. Commun. 19, 383, (1965)
MerckMerck 13,5659